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Cell Line
SCLC-21H Cell Line
tccc302206
SCLC-21H Cell Line
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AVAILABLE SIZES
1x10^6 Cells/Vial
$
0.00
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
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Western blot (WB)
Immunohistochemistry (IHC)
Immunofluorescence (IF)
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Immunoprecipitation (IP)
Co-Immunoprecipitation (CoIP)
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RNA Binding Protein Immunoprecipitation (RIP)
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Product Description
Human Lung small cell carcinoma cell line SCLC-21H
Information
Host Species
Homo sapiens (human)
Cell Name
Human Lung small cell carcinoma cell line SCLC-21H
Cell Type: Small cell lung carcinoma
Tissue
Lung (pleural effusion)
Morphology
Large cell-like
Growth Properties
Suspension, floating cell aggregates
Specifications
Form
Frozen cells
Subcultivation Ratio
A ratio of 1:2 to 1:4 is recommended
Culture Conditions
RPMI 1640 medium supplemented with 2 mM L-glutamine, sodium pyruvate, and 10% fetal bovine serum
Misc Information
Protocol
Seeding Density: Start cultures at 2-4x10e4 cells/ml
Fluid Renewal: 2 to 3 times weekly
Doubling Time: 45 hrs
Subculturing
Once or twice a week add 5 ml of fresh cell culture medium, as soon as the culture medium gets acidic. Suculture as soon as many very large clusters are observed. Dissociate the clusters by collecting the cells, rinsing once using PBS without calcium/magnesium and adding 3-5 ml Accutase. Incubate for 10minutes at 37°C. Collect the cells following centrigation, resuspend in fresh cell culture medium and count
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