tcel440 SK-MEL-1

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Product Description

Electron microscopy revealed pigment granules relating both to synthesis and to phagocytosis. Antibody to this line was detected in 63% of patients with malignant melanoma and in 10% of patients with other diseases.

Information

ApplicationAntibody to this line was detected in 63% of patients with malignant melanoma and in 10% of patients with other diseases. Electron microscopy revealed pigment granules relating both to synthesis and to phagocytosis.
TissueHuman malignant melanoma
skin
derived from metastatic site: lymphatic system
Morphologyspherical
Growth Propertiesclusters in suspension
EffectsYes, in nude mice forms pigmented malignant melanomas
also forms tumors in the cheek pouch of cortisone treated hamsters.

Specifications

Complete Growth MediumMEM (PM150410)+10% FBS (164210-500)+1% P/S (PB180120)
Subcultivation Ratio1:2-1:4
Medium Renewalevery 2 to 3 days
CryopreservationFreeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture ConditionsAtmosphere: Air, 95%; CO2, 5% Temperature: 37℃
TumorigenicYes
Antigen ExpressionBlood Type A; Rh+
DurationAntibody to this line was detected in 63% of patients with malignant melanoma and in 10% of patients with other diseases. Electron microscopy revealed pigment granules relating both to synthesis and to phagocytosis.

Misc Information

SubculturingCultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at the subcultivation ratio of 1:2-1:4.
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