tcel328 CEM / C1

Order Now

AVAILABLE SIZES

$0.00 ORDERING INFORMATION International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807

Data sheet

Download PDF Datasheet

Technical Inquries

Submit Review

Product Description

This cell line exhibits cross resistance to both the water soluble(topotecan) and water insoluble(9-amino-CPT and 10,11-methylenedioxy-CPT) analogs of CPT.CEM/C1 cells are approximately 31 fold less sensitive to CPT than the parental CEM cells.CEM/C1 cells display atypical multidrug resistance(MDR) and altered topoisomerase I catalytic activity.Resistance to CPT is stable for up to six months.

Information

TissueHuman peripheral blood
Morphologylymphoblast
Growth Propertiessuspension

Specifications

Complete Growth MediumRPMI-1640 (PM150110)+10% FBS (164210-500)+1% P/S (PB180120)
Subcultivation Ratio1:2-1:4
Medium Renewalevery 2 to 3 days
CryopreservationFreeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture ConditionsAtmosphere: Air, 95%; CO2, 5% Temperature: 37℃

Misc Information

SubculturingCultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at the subcultivation ratio of 1:2-1:4.
Get valuable resources and offers directly to your email.