tcel149 MCF7 [MCF-7]

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Product Description

The MCF7 line retains several characteristics of differentiated mammary epithelium including ability to process Estradiol via cytoplasmic estrogen receptors and the capability of forming domes. The cells express the WNT7B oncogene.RefGrowth of MCF7 cells is inhibited by tumor necrosis factor alpha(TNF alpha).Secretion of IGFBP's can be modulated by treatment with anti-estrogens.


TissueHuman mammary gland, breast
derived from metastatic site: pleural effusion
Growth Propertiesadherent


Complete Growth MediumMEM (PM150410)+0.01mg/mL rh-Insulin (PB180433)+10% FBS (164210-500)+1% P/S (PB180120)
Subcultivation Ratio1:2-1:4
Medium Renewalevery 2 to 3 days
CryopreservationFreeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture ConditionsAtmosphere: Air, 95%; CO2, 5% Temperature: 37℃

Misc Information

SubculturingRemove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
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