Product Description
CCRF-CEM cells were derived from the peripheral blood buffy coat of a child(CEM) with acute lymphoblastic leukemia who had originally presented with lymphosarcoma. Do not centrifuge after thawing.
Information
Applicationtransfection host
TissueHuman peripheral blood
Morphologylymphoblast
Growth Propertiessuspension
EffectsYes, in syrian hamsters.
Specifications
Complete Growth MediumRPMI-1640 (PM150110)+10% FBS (164210-500)+1% P/S (PB180120)
Subcultivation Ratio1:2-1:4
Medium Renewalevery 2 to 3 days
CryopreservationFreeze medium: 60% Basal medium＋30% FBS＋10% DMSO
Storage temperature: Liquid nitrogen vapor phase
Culture ConditionsAtmosphere: Air, 95%; CO2, 5%
Temperature: 37℃
TumorigenicYes
Antigen ExpressionCD3; Homo sapiens, expressed; CD4; Homo sapiens, expressed; CD5; Homo sapiens, expressed; CD7; Homo sapiens, expressed
Gene ExpressionCD3; Homo sapiens, expressed , CD5; Homo sapiens, expressed , CD7; Homo sapiens, expressed , CD4; Homo sapiens, expressed
Durationtransfection host
Misc Information
SubculturingCultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at the subcultivation ratio of 1:3-1:5.