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Product Description
The cells are EBNA negative and are negative for Fc receptors.Approximately 12% of the cells express commplement receptors.
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Information
TissueHuman lymphoblast
Morphologylymphoblast
Growth Propertiessuspension
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Specifications
Complete Growth MediumRPMI-1640 (PM150110)+20% FBS (164210-500)+1% P/S (PB180120)
Subcultivation Ratio1:2-1:4
Medium Renewalevery 2 to 3 days
CryopreservationFreeze medium: 60% Basal medium+30% FBS+10% DMSO
Storage temperature: Liquid nitrogen vapor phase
Culture ConditionsAtmosphere: Air, 95%; CO2, 5%
Temperature: 37℃
Receptor Expressioncomplement
Antigen ExpressionHLA B27(the patient was HLA A2, A11, B17, B27)
Gene Expressionimmunoglobulin(surface and secreted)
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Misc Information
SubculturingCultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2×10^5 viable cells/ml. Maintain cell density between 1×10^5 and 1×10^6 viable cells/ml.