Product Description
The cells are EBNA negative and are negative for Fc receptors.Approximately 12% of the cells express commplement receptors.
Information
TissueHuman lymphoblast
Morphologylymphoblast
Growth Propertiessuspension
Specifications
Complete Growth MediumRPMI-1640 (PM150110)+20% FBS (164210-500)+1% P/S (PB180120)
Subcultivation Ratio1:2-1:4
Medium Renewalevery 2 to 3 days
CryopreservationFreeze medium: 60% Basal medium＋30% FBS＋10% DMSO
Storage temperature: Liquid nitrogen vapor phase
Culture ConditionsAtmosphere: Air, 95%; CO2, 5%
Temperature: 37℃
Receptor Expressioncomplement
Antigen ExpressionHLA B27(the patient was HLA A2, A11, B17, B27)
Gene Expressionimmunoglobulin(surface and secreted)
Misc Information
SubculturingCultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2×10^5 viable cells/ml. Maintain cell density between 1×10^5 and 1×10^6 viable cells/ml.