tcel37 BNL CL.2

Order Now

AVAILABLE SIZES

$0.00 ORDERING INFORMATION International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807

Data sheet

Download PDF Datasheet

Technical Inquries

Submit Review

Product Description

The line was selected to grow in medium containing ornithine and phenylalanine in place of arginine and tyrosine.Tested and found negative for ectromelia virus(mousepox).

Information

ApplicationControl for F4 G6PD Knockout.
TissueMouse liver
Morphologyepithelial
Growth Propertiesadherent

Specifications

Complete Growth MediumDMEM (PM150210)+10% FBS (164210-500)+1% P/S (PB180120)
Subcultivation Ratio1:2-1:4
Medium Renewalevery 2 to 3 days
CryopreservationFreeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture ConditionsAtmosphere: Air, 95%; CO2, 5% Temperature: 37℃
TumorigenicNo, the cells were not tumorigenic in immunosuppressed mice, but did form colonies in semisolid medium.
DurationControl for F4 G6PD Knockout.

Misc Information

SubculturingRemove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Get valuable resources and offers directly to your email.