tcel28 AtT-20

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Product Description
Tested and found negative for ectromelia virus(mousepox).

Information
Applicationtransfection host
TissueMouse pituitary
Morphologysmall rounded cells
Growth Propertiesmixed, adherent and suspension

Specifications
Complete Growth MediumHam's F-12K (PM150910)+15% HS (164215-100)+2.5% FBS (164210-500)+1% P/S (PB180120)
Subcultivation Ratio1:2-1:4
Medium Renewal2 to 3 times per week
CryopreservationFreeze medium: 60% Basal medium＋30% FBS＋10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture ConditionsAtmosphere: Air, 95%; CO2, 5% Temperature: 37℃
Gene Expressionadrenocorticotropic hormone(ACTH)
Durationtransfection host

Misc Information
SubculturingRemove and discard culture medium. These cells grow as a mixture of floating and adherent cells. Sometimes many cells are floating, they can be harvested by centrifugation of medium instead of discarding it. Add 1.0 to 2.0 mL of 0.25% (w/v) Trypsin-0.53mM EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C.
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