tcel10 786-O [786-0]

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Product Description

The cells display both microvilli and desmosomes, and can be grown in soft agar.(PubMed: 2835539)The cells produce a PTH like peptides that is identical to peptides produced by breast and lung tumors.The peptide has an N terminal sequence similar to PTH, has PTH like activity, and has a molecular weight of 6000 daltons.

Information

Applicationtransfection host
TissueHuman kidney
Morphologyepithelial
Growth Propertiesadherent
EffectsYes, in immunosuppressed hamsters.

Specifications

Complete Growth MediumRPMI-1640 (PM150110)+10% FBS (164210-500)+1% P/S (PB180120)
Subcultivation Ratio1:3-1:6
Medium Renewalevery 2 to 3 days
CryopreservationFreeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture ConditionsAtmosphere: Air, 95%; CO2, 5% Temperature: 37℃
TumorigenicYes
Gene Expressionparathyroid hormone(PTH) like peptide
Durationtransfection host

Misc Information

SubculturingRemove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
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