tcna4755 Recombinant Goat Anti-Mouse IgG Fab Antibody

ELISA of mouse immunoglobulins shows the recombinant Mouse IgG Fab antibody reacts to the Fab region of mouse IgG1, IgG2a, IgG2b, and IgG3; no cross reactivity with IgM, IgA, IgE, human/rat/rabbit IgG.ELISA Titration: the plate was coated with different amounts of mouse IgG. A serial dilution of recombinant Mouse IgG Fab antibody was used as the primary and an alkaline phosphatase conjugated anti-goat IgG as the secondary.
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Product Description
This recombinant Mouse IgG Fab antibody reacts to the Fab region of Mouse IgG, including IgG1, IgG2a, IgG2b, and IgG3. No cross reactivity with IgM, IgA, IgE, human IgG, rat IgG, or rabbit IgG.

Information
ApplicationICC, IHC, FACS, ELISA
Species ReactivityMouse
Host SpeciesGoat
Immunogen / Amino acidsMouse IgG was used as the immunogen for this recombinant Mouse IgG Fab antibody.
ConjugationBiotin Conjugate
ClonalityRecombinant Goat Monoclonal
ClonesRMG05
IsotypeGoat IgG

Specifications
FormLiquid
Storage Buffer1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Recommended DilutionELISA: 0.05ug/ml - 1ug/ml
Immunocytochemistry: 0.5-2ug/ml
Immunohistochemistry (FFPE): 0.5-2ug/ml (1)The stated application concentrations are suggested starting points. Titration of the recombinant Mouse IgG Fab antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Misc Information
Storage InstructionStore the recombinant Mouse IgG Fab antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).
Gene IDN/A (human);
ReferencesBiotin Conjugate
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