tcna4753 Recombinant Goat Anti-Rabbit IgG Fab Antibody

ELISA of IgGs from different species shows the recombinant Rabbit IgG Fab antibody reacts to the Fab region of rabbit IgG; no cross reactivity with human IgG, rat IgG, or mouse IgG.Western blot of nonreduced(-) and reduced(+) rabbit IgG (20ng/lane), using 0.2ug/ml of recombinant Rabbit IgG Fab antibody. This antibody reacts to nonreduced rabbit IgG (~150 kDa).ELISA Titration: the plate was coated with different amounts of rabbit IgG. A serial dilution of the recombinant Rabbit IgG Fab antibody was used as the primary and an alkaline phosphatase conjugated anti-goat IgG as the secondary.
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Product Description
This recombinant Rabbit IgG Fab antibody reacts to the Fab region of Rabbit IgG. No cross reactivity with human IgG, rat IgG, or mouse IgG

Information
ApplicationWB (non-reduced), ICC, IHC, FACS, ELISA
Species ReactivityRabbit
Host SpeciesGoat
Immunogen / Amino acidsRabbit IgG was used as the immunogen for this recombinant Rabbit IgG Fab antibody.
ConjugationBiotin Conjugate
ClonalityRecombinant Goat Monoclonal
ClonesRMG01
IsotypeGoat IgG

Specifications
FormLiquid
Storage Buffer1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Recommended DilutionELISA: 0.01ug/ml - 0.5ug/ml
Immunocytochemistry: 0.5-2ug/ml
Immunohistochemistry (FFPE): 0.5-2ug/ml (1)
Western Blot (non-reduced): 0.1-0.5ug/mlThe stated application concentrations are suggested starting points. Titration of the recombinant Rabbit IgG Fab antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Misc Information
Storage InstructionStore the recombinant Rabbit IgG Fab antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).
Gene IDN/A (human);
ReferencesBiotin Conjugate
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