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Secondary Antibody
Rabbit Anti-Human IgG Heavy Chain Antibody- prediluted
tcna1486pre
Rabbit Anti-Human IgG Heavy Chain Antibody- prediluted
IHC staining of FFPE human tonsil tissue with recombinant IgG antibody (clone GHC07-2R). Required HIER: boil tissue sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.
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AVAILABLE SIZES
7ml
$
526.00
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
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Application
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Western blot (WB)
Immunohistochemistry (IHC)
Immunofluorescence (IF)
Immunocytochemistry (ICC)
Immunoprecipitation (IP)
Co-Immunoprecipitation (CoIP)
Chromatin Immunoprecipitation (ChIP)
RNA Binding Protein Immunoprecipitation (RIP)
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UG
CELLS
Treatment
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Product Description
This antibody reacts with all sub-classes of gamma chain of human immunoglobulins. It does not cross-react with alpha (IgA), mu (IgM), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. It is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.
Information
Application
IHC-P
Species Reactivity
Human. Other species not tested.
Host Species
Rabbit
Immunogen / Amino acids
Human Ig Gamma Chain was used as the immunogen for this recombinant IgG antibody.
Conjugation
Unconjugated
Clonality
Recombinant Rabbit Monoclonal
Clones
GHC07-2R
Isotype
Rabbit IgG, kappa
Specifications
Form
Liquid
Storage Buffer
Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*
Recommended Dilution
Prediluted IHC only format : incubate for 30 min at RT (1)The stated application concentrations are suggested starting points. Titration of the recombinant IgG antibody may be required due to differences in protocols and secondary/substrate sensitivity.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required)
drip mAb solution onto the tissue section and incubate at RT for 30 min.
Misc Information
Storage Instruction
Store the recombinant IgG antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
SwissProt
P01857
References
Protein A affinity chromatography
related data
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