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Secondary Antibody
Mouse Anti-Human IgG Heavy Chain Antibody- prediluted
tcna1381pre
Mouse Anti-Human IgG Heavy Chain Antibody- prediluted
IHC testing of FFPE human tonsil with recombinant human IgG antibody (clone rIG266). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
SDS-PAGE analysis of purified, BSA-free recombinant human IgG antibody (clone rIG266) as confirmation of integrity and purity.
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AVAILABLE SIZES
7ml
$
526.00
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
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Western blot (WB)
Immunohistochemistry (IHC)
Immunofluorescence (IF)
Immunocytochemistry (ICC)
Immunoprecipitation (IP)
Co-Immunoprecipitation (CoIP)
Chromatin Immunoprecipitation (ChIP)
RNA Binding Protein Immunoprecipitation (RIP)
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Product Description
Recognizes a protein of 75kDa, identified as gamma heavy chain of human immunoglobulins. It reacts with all sub-classes of gamma chain of human immunoglobulins. It does not cross-react with alpha (IgA), mu (IgM), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This mAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.
Information
Application
IHC-P
Species Reactivity
Human. Other species not known.
Host Species
Mouse
Immunogen / Amino acids
Human Ig Gamma Chain was used as the immunogen for this recombinant human IgG antibody.
Conjugation
Unconjugated
Clonality
Recombinant Mouse Monoclonal
Clones
rIG266
Isotype
Mouse IgG1, kappa
Specifications
Form
Liquid
Storage Buffer
Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*
Recommended Dilution
Prediluted IHC only format : incubate for 30 min at RT (1)The optimal dilution of the recombinant human IgG antibody for each application should be determined by the researcher.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required)
drip mAb solution onto the tissue section and incubate at RT for 30 min.
Misc Information
Storage Instruction
Store the recombinant human IgG antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
SwissProt
P01857
References
Protein G affinity chromatography
related data
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