tcba13398 Phospho-ERK1-T202 / Y204 + ERK2-T185 / Y187 pAb

Western blot analysis of extracts of Jurkat cells, using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 antibody at 1:2000 dilution. Jurkat cells were treated by PMA/TPA (200nM) for 10 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 5s.Immunoprecipitation - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb.Western blot analysis of extracts of various cell lines, using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 antibody at 1:5000 dilution or ERK1/2 antibody .293T cells and C6 cells were treated by ATP(5 mM) at 30℃ for 1 hour. NIH/3T3 cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.Immunohistochemistry of paraffin-embedded rat lung using Phospho-ERK1-T202-Y204 + ERK2-T185-Y187 antibody (tcba13398) at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded rat testis using Phospho-ERK1-T202-Y204 + ERK2-T185-Y187 antibody (tcba13398) at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded rat brain using Phospho-ERK1-T202-Y204 + ERK2-T185-Y187 antibody (tcba13398) at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded rat spleen using Phospho-ERK1-T202-Y204 + ERK2-T185-Y187 antibody (tcba13398) at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded rat heart using Phospho-ERK1-T202-Y204 + ERK2-T185-Y187 antibody (tcba13398) at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human tonsil using Phospho-ERK1-T202-Y204 + ERK2-T185-Y187 antibody (tcba13398) at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human breast cancer using Phospho-ERK1-T202-Y204 + ERK2-T185-Y187 antibody (tcba13398) at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human gastric cancer using Phospho-ERK1-T202-Y204 + ERK2-T185-Y187 antibody (tcba13398) at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded human uterine cancer using Phospho-ERK1-T202-Y204 + ERK2-T185-Y187 antibody (tcba13398) at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse liver using Phospho-ERK1-T202-Y204 + ERK2-T185-Y187 antibody (tcba13398) at dilution of 1:100 (40x lens).Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (AP0472) Immunohistochemistry of paraffin-embedded mouse testis using Phospho-ERK1-T202-Y204 + ERK2-T185-Y187 antibody (tcba13398) at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse brain using Phospho-ERK1-T202-Y204 + ERK2-T185-Y187 antibody (tcba13398) at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse spleen using Phospho-ERK1-T202-Y204 + ERK2-T185-Y187 antibody (tcba13398) at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse kidney using Phospho-ERK1-T202-Y204 + ERK2-T185-Y187 antibody (tcba13398) at dilution of 1:100 (40x lens).Immunohistochemistry of paraffin-embedded mouse heart using Phospho-ERK1-T202-Y204 + ERK2-T185-Y187 antibody (AP0472) at dilution of 1:100 (40x lens).
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Product Description
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Information
ApplicationWB, IP, IHC
Research AreaCancer, MAPK pathway, Neuroscience, Immunology, Innate Immunity, Neurodegeneration,
Species ReactivityHuman, Mouse, Rat
Host SpeciesRabbit
Immunogen / Amino acidsA synthetic phosphorylated peptide around T185/Y187 of human ERK2 (NP_002736.3).
IsotypeIgG

Specifications
FormLiquid
Storage BufferBuffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended DilutionWB 1:500 - 1:2000
IP 1:50 - 1:100

Misc Information
Storage InstructionStore at -20℃. Avoid freeze / thaw cycles.
SwissProtP28482 / P27361
Gene ID5594/5595 (human);
Reference Sequence No.FLTEYVA
Calculated Molecular Weight36kDa/41kDa/38kDa/40kDa/43kDa
PurificationAffinity purification
ReferencesWB(MDA-MB-231, Raw264.7, Mouse splenocytes, C6, Murine, Human, Mouse, Rat, Pig) IF(Human)
Positive ControlJurkat
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