tcip3012 EBOV Delta Peptide

Human promyelocytic leukemia cell line HL60 was differentiated into neutrophils by treatment with DMSO. Neutrophils were incubated with serial dilutions of LukA-LukB for 3 hours at 37°C with 5% CO2 and 95% humidity.; Cellular viability was determined by adding XTT and incubating for additional 16 hours. Cells were centrifuged and the OD determined in the supernatants at 470/690 nm. EC 50 value was determined to be 0.111 nM.DS-PAGE (Panel A); 1 µg and 5 µg of LukA-LukB were loaded under denaturing and reducing condition (lanes 1 and 2). MW denotes Novex Shar prestained protein markers. ; Western Blots (Panels B and C); Heat-denatured and reduced LukA-LukB was loaded at 200 ng, 100 ng and 50 ng (lanes 1, 2 and 3). In Panel (B), LukA was detected using 0.5 µg/mL of rabbit anti- LukA polyclonal antibody (Cat# tcia162). In Panel (C), LukB was detected using 0.5 µg/mL of rabbit anti-LukB polyclonal antibody (Cat# tcia163). An anti-rabbit IgG-HRP conjugate was then added, followed by TMB Membrane Substrate.
Order Now

AVAILABLE SIZES

 $0.00 ORDERING INFORMATION International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807

Data sheet

Download PDF Datasheet

Technical Inquries

Submit Review

Product Description
Purified Ebolavirus Delta Peptide.

Information
ApplicationWB, ELISA
Research AreaVirology

Specifications
FormFrozen Liquid
ConcentrationSupplied in PBS at a concentration of 0.910 mg/mL (Lot dependent)
Purity / GradeColumn chromatography

Misc Information
Storage Instruction2-3 weeks at -20°C, -80°C long term
Get valuable resources and offers directly to your email.
COMPANY About Taiclone Shipping Terms Taiclone Guarantee
RESOURCES Life Science Glossary
PRODUCTS Primary AntibodySecondary AntibodyProtein & PeptidesBioChemicalsAssay & Elisa KitsLoading ControlOther ReagentsSerumsCell Line
SUPPORT Contact Us Email Us Terms of Service Privacy Policy
Copyright 2016-2023 Taiclone Biotech Corp. All Rights Reserved.