tcue3044 ELISA Kit for Kallikrein 14 (KLK14)

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Data sheet

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Test Principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Kallikrein 14 (KLK14) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Kallikrein 14 (KLK14) and unlabeled Kallikrein 14 (KLK14) (Standards or samples) with the pre-coated antibody specific to Kallikrein 14 (KLK14). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Kallikrein 14 (KLK14) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Kallikrein 14 (KLK14) in the sample.

Information

Research AreaEnzyme & Kinase
Species ReactivityHomo sapiens (Human)
Sample TypeSerum, plasma, tissue homogenates and other biological fluids.

Specifications

Tested ApplicationELISA
SensitivityThe minimum detectable dose of this kit is typically less than 43.2pg/mL
Detection Range123.46-10000pg/mL
Assay Time2h
Detection MethodEnzyme-linked immunosorbent assay for Antigen Detection.

Misc Information

SwissProtQ9P0G3
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