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Assay & Elisa Kits
PGE2 (Prostaglandin E2) ELISA Kit
tcee5213
PGE2 (Prostaglandin E2) ELISA Kit
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AVAILABLE SIZES
96T
$
520.00
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
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Product Description
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with PGE2. During the reaction, PGE2 in the sample or standard competes with a fixed amount of PGE2 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to PGE2. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PGE2 in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Information
Application
This ELISA kit applies to the in vitro quantitative determination of PGE2 concentrations in serum, plasma and other biological fluids.
Species Reactivity
Universal
Sample Type
serum, plasma and other biological fluids
50μL
Assay Type
Competitive
Specifications
Tested Application
ELISA
Sensitivity
18.75pg/mL
Detection Range
31.25~2000pg/mL
Assay Time
2.5h
Detection Method
Competitive-ELISA
Misc Information
Storage Instruction
An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the detailed information on the manual once the kit is received
Notes
Specificity:This kit recognizes PGE2 in samples. No significant cross-reactivity or interference between PGE2 and analogues was observed.
Both intra-CV and inter-CV are < 10%
related data
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