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Loading Control
anti β Tubulin Loading Control Antibody
tcaba2
anti β Tubulin Loading Control Antibody
Fig. 1 Three loading control mAbs reacting against 10μg/lane of mouse brain tissue lysates. 50kDa band is Anti-βTubulin (BT7R) at 1:2000 dilution (0.5μg/mL); 42kDa band is Anti-βActin (BA3R) at 1:1000 dilution (1μg/mL); 37kDa band is AntiGAPDH (GA1R) at 1:5000 dilution (0.2μg/mL)
Fig. 02 1:4000 (0.25μg/mL) Ab dilution used in WB of 10μg/lane mouse brain tissue lysates
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AVAILABLE SIZES
0.1mg
$
299.00
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
Data sheet
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Application
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Western blot (WB)
Immunohistochemistry (IHC)
Immunofluorescence (IF)
Immunocytochemistry (ICC)
Immunoprecipitation (IP)
Co-Immunoprecipitation (CoIP)
Chromatin Immunoprecipitation (ChIP)
RNA Binding Protein Immunoprecipitation (RIP)
Sample
Loading Amount
Loading Type
UG
CELLS
Treatment
Temperature
Exposure
Observe Band
Fixative
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Acetone
Formaldehyde
Methanol
Paraformaldehyde
Other
Antigen Retrieval
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Heat mediated
Enzymatic
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Positive Control
Negative Control
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Product Description
No product descriptions available. Please refer to the product information & specifications for details.
Information
Application
ELISA, Immunostaining, Western Blotting, Dot Blot
Research Area
loading control
Species Reactivity
human, monkey, mouse, rat, rabbit, chicken
Host Species
Mouse
Conjugation
Unconjugated
Clonality
Monoclonal Antibody
Clones
BT7R
Isotype
Mouse IgG2a
Specifications
Form
Liquid
Storage Buffer
PBS, Stabilizers, 0.05% ProClin 300
Concentration
1mg/mL
Recommended Dilution
WB (ECL): 1:2
000-5
000 dilution (incubate for 1 hour at room
temperature)
IS: 1:500-2000 dilution
Misc Information
Storage Instruction
Avoid repeated freeze/thaw cycles. Aliquot and store at -20 ℃ for 12 months.
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