Product Description
HULEC-5a, has been shown to retain many of the characteristics of endothelial cells. These immortalized cells, because they are a continously renewable source of human endothelial microvascular cells, can be used as a replacement for primary human lung endothelial cells for many research studies.
Information
ApplicationInvestigating the role of endothelium in infection by virus and bacteria, cancer research, tumor metastastis, endothelium function, cell trafficking, surface molecule interactions
TissueHuman lung/vascular endothelium
Morphologyepithelial
Growth Propertiesadherent
Specifications
Complete Growth MediumMCDB 131(without L-Glutamine) (PM151210)+10ng/mL EGF+1μg/mL Hydrocortisone+10mM Glutamine (PB180420)+10% FBS (164210-500)+1% P/S (PB180120)
Subcultivation Ratio1:2-1:4
Medium Renewalevery 2 to 3 days
CryopreservationFreeze medium: 60% Basal medium＋30% FBS＋10% DMSO
Storage temperature: Liquid nitrogen vapor phase
Culture ConditionsAtmosphere: Air, 95%; CO2, 5%
Temperature: 37℃
Receptor Expressionneonatal FcRn receptor (FcRn)
DurationInvestigating the role of endothelium in infection by virus and bacteria, cancer research, tumor metastastis, endothelium function, cell trafficking, surface molecule interactions
Misc Information
SubculturingRemove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor.
Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.