tcel470 SV40 MES 13

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Product Description

The cells form colonies in soft agar, and are positive for SV40 large T antigen.The cells display prominent cytoskeletal staining for actin, and have abundant parallel fibrils in the cytoplasm. They are reported to contract in the presence of 1×10^-6 M angiotensin Ⅱ. The cells form colonies in soft agar, and are positive for SV40 large T antigen.

Information

TissueMouse kidney/glomerulus
Morphologymyofibroblast-like
Growth Propertiesadherent
EffectsYes, forms colonies in soft agar.

Specifications

Complete Growth Medium71.25% DMEM (PM150210)+23.75% F12 (PM150810)+5% FBS (164210-500)+1% P/S (PB180120)
Subcultivation Ratio1:2-1:4
Medium Renewalevery 2 to 3 days
CryopreservationFreeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture ConditionsAtmosphere: Air, 95%; CO2, 5% Temperature: 37℃

Misc Information

SubculturingRemove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
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