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Cell Line
SK-NEP-1
tcel442
SK-NEP-1
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AVAILABLE SIZES
1×10⁶cells/t25culturebottle
$
0.00
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
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Western blot (WB)
Immunohistochemistry (IHC)
Immunofluorescence (IF)
Immunocytochemistry (ICC)
Immunoprecipitation (IP)
Co-Immunoprecipitation (CoIP)
Chromatin Immunoprecipitation (ChIP)
RNA Binding Protein Immunoprecipitation (RIP)
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UG
CELLS
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Acetone
Formaldehyde
Methanol
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Heat mediated
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Product Description
Ultrastructural features include few microvilli, junctional complexes, well formed Golgi, mostly smooth ER, lipid droplets, no virus particles.
Information
Tissue
Human kidney
derived from metastatic site: pleural effusion
Morphology
rounded
Growth Properties
suspension
Effects
Yes, in nude mice forms tumor with small cells consistent with Wilms'tumor.
Specifications
Complete Growth Medium
McCoy's 5A (PM150710)+15% FBS (164210-500)+1% P/S (PB180120)
Subcultivation Ratio
1:2-1:4
Medium Renewal
every 2 to 3 days
Cryopreservation
Freeze medium: 60% Basal medium＋30% FBS＋10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture Conditions
Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
Tumorigenic
Yes
Misc Information
Subculturing
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at the subcultivation ratio of 1:3-1:6.
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