Product Description
Ultrastructural studies demonstrated the presence of cytoplasmic structures characteristic of Clara cells.The cells expressed protein and RNA of SP-A, the major lung surfactant associated protein.SP-B and SP-C RNA was not expressed.They have a reported colony forming efficiency of 0.83% in soft agarose.
Information
ApplicationThis line was derived by A.F.Gazdar, H.K.Oie, J.D.Minna and associates in 1981 from tumor tissue obtained from a patient prior to initiation of chemotherapy. Ultrastructural studies demonstrated the presence of cytoplasmic structures characteristic of Cla
TissueHuman lung/bronchiole
derived from metastatic site: alveolus
Morphologyepithelial
Growth Propertiesadherent
EffectsYes, the cells produce tumors in athymic nude mice.
Specifications
Complete Growth MediumRPMI-1640 (PM150110)+10% FBS (164210-500)+1% P/S (PB180120)
Subcultivation Ratio1:2-1:4
Medium Renewalevery 2 to 3 days
CryopreservationFreeze medium: 60% Basal medium＋30% FBS＋10% DMSO
Storage temperature: Liquid nitrogen vapor phase
Culture ConditionsAtmosphere: Air, 95%; CO2, 5%
Temperature: 37℃
TumorigenicYes
Gene Expressionlung surfactant associated protein A(SP-A), The cells expressed protein and RNA of SP-A, the major lung surfactant associated protein.
DurationThis line was derived by A.F.Gazdar, H.K.Oie, J.D.Minna and associates in 1981 from tumor tissue obtained from a patient prior to initiation of chemotherapy. Ultrastructural studies demonstrated the presence of cytoplasmic structures characteristic of Cla
Misc Information
SubculturingRemove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor.
Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.