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Cell Line
MDA-MB-436
tcel383
MDA-MB-436
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AVAILABLE SIZES
1×10⁶cells/t25culturebottle
$
0.00
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
Data sheet
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Application
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Western blot (WB)
Immunohistochemistry (IHC)
Immunofluorescence (IF)
Immunocytochemistry (ICC)
Immunoprecipitation (IP)
Co-Immunoprecipitation (CoIP)
Chromatin Immunoprecipitation (ChIP)
RNA Binding Protein Immunoprecipitation (RIP)
Sample
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UG
CELLS
Treatment
Temperature
Exposure
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Fixative
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Acetone
Formaldehyde
Methanol
Paraformaldehyde
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Antigen Retrieval
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Heat mediated
Enzymatic
Other
None
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Product Description
The line is pleomorphic and most cells react intensely with anti tubulin antibody as demonstrated by indirect immunofluorescence staining.
Information
Tissue
Human mammary gland/breast
derived from metastatic site: pleural effusion
Morphology
pleomorphic with multinucleated component cells
Growth Properties
adherent
Effects
No, in immunosuppressed mice
Yes, in semisolid medium.
Specifications
Complete Growth Medium
DMEM (PM150210)+10μg/mL Insulin (PB180433)+16μg/mL Glutathione+15% FBS (164210-500)+1% P/S (PB180120)
Subcultivation Ratio
1:2-1:4
Medium Renewal
every 2 to 3 days
Cryopreservation
Freeze medium: 60% Basal medium＋30% FBS＋10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture Conditions
Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
Tumorigenic
No
Gene Expression
tubulin; actin
Misc Information
Subculturing
Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
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