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Cell Line
MDA-MB-415
tcel382
MDA-MB-415
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AVAILABLE SIZES
1×10⁶cells/t25culturebottle
$
0.00
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
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Western blot (WB)
Immunohistochemistry (IHC)
Immunofluorescence (IF)
Immunocytochemistry (ICC)
Immunoprecipitation (IP)
Co-Immunoprecipitation (CoIP)
Chromatin Immunoprecipitation (ChIP)
RNA Binding Protein Immunoprecipitation (RIP)
Sample
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UG
CELLS
Treatment
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Heat mediated
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Product Description
The cells express the WNT7B oncogene [PubMed: 8168088].The patient presenting with the tumor was from Paraguay and, although listed as Caucasian, may have had mixed ancestry as suggested by the presence of G6PD type A phenotype in the cells.The line forms flat, spreading plaques of epithelia which exhibit desmosomes, extensive microtubules and microfilaments upon examination with the electron microscope. It is not amenable to trypsinization.
Information
Tissue
Human mammary gland/breast
derived from metastatic site: pleural effusion
Morphology
epithelial
Growth Properties
adherent
Effects
Yes, in semisolid medium
No, in immunosuppressed mice.
Specifications
Complete Growth Medium
DMEM (PM150210)+10μg/mL Insulin (PB180433)+10μg/mL Glutathione+15% FBS (164210-500)+1% P/S (PB180120)
Subcultivation Ratio
1:2-1:4
Medium Renewal
every 2 to 3 days
Cryopreservation
Freeze medium: 60% Basal medium＋30% FBS＋10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture Conditions
Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
Tumorigenic
No
Antigen Expression
Blood Type O
Gene Expression
amelogenin(X chromosome)(AMELEX)
Misc Information
Subculturing
Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
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