tcel307 SW1116

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Product Description

CSAp negative(CSAp-).Colon antigen 3, negative.The cells are positive for keratin by immunoperoxidase staining.The line is positive for expression of c-myc, K-ras, H-ras, myb, sis and fos oncogenes.N-myc and N-ras oncogene expression were not detected.Tumor specific nuclear matrix proteins CC-4, CC-5 and CC-6 are expressed.

Information

TissueHuman colon
Morphologyepithelial
Growth Propertiesadherent
EffectsYes, in nude mice. Tumors developed within 21 days at 100% frequency(5/5) in nude mice inoculated subcutaneously with 10^7 cells.

Specifications

Complete Growth MediumDMEM (PM150210)+10% FBS (164210-500)+1% P/S (PB180120)
Subcultivation Ratio1:2-1:4
Medium Renewalevery 2 to 3 days
CryopreservationFreeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture ConditionsAtmosphere: Air, 95%; CO2, 5% Temperature: 37℃
TumorigenicYes
Antigen ExpressionBlood Type O; Rh+
Gene Expressioncarcinoembryonic antigen(CEA) 2654ng/10^6 cells/10 days; keratin

Misc Information

SubculturingCells must be subcultured at about 80% confuency , before they reach 90%. Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
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