Product Description
Although the tissue donor was heterozygous for the G6PD alleles, the cell line consistently showed only the G6PD A phenotype. There is a G-】 A mutation in codon 273 of the p53 gene resulting in an Arg-】 His substitution. EGF receptor is present at 1×10^6 per cell.
Information
Applicationtransfection host
TissueHuman mammary gland/breast
derived from metastatic site: pleural effusion
Morphologyepithelial
Growth Propertiesadherent
EffectsYes, in nude mice inoculated subcutaneously with 10^7 cells. Tumors developed within 21 days at 100% frequency(5/5).
Specifications
Complete Growth MediumDMEM (PM150210)+10% FBS (164210-500)+1% P/S (PB180120)
Subcultivation Ratio1:2-1:4
Medium Renewalevery 2 to 3 days
CryopreservationFreeze medium: 60% Basal medium＋30% FBS＋10% DMSO
Storage temperature: Liquid nitrogen vapor phase
Culture ConditionsAtmosphere: Air, 95%; CO2, 5%
Temperature: 37℃
TumorigenicYes
Receptor Expressionepidermal growth factor(EGF); transforming growth factor alpha(TGF alpha)
Antigen ExpressionBlood Type AB; HLA Aw23, Aw30, B27, Bw35, Cw2, Cw4(patient)
Durationtransfection host
Misc Information
SubculturingRemove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor.
Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.