tcel196 RKO

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Product Description

RKO cells contain wild-type p53 but lack endogenous human thyroid receptor nuclear receptor(h-TRbeta1). The level of p53 protein is higher in RKO cells than in RKO-E6 cells. The RKO cell line is the parental cell line(isogenic) of RKO-E6 and RKO-A545-1.

Information

ApplicationIt can be used as the control cell line for investigating the effects of p53 and gadd45 on cellular parameters.
TissueHuman colon
Morphologyepithelial
Growth Propertiesadherent
EffectsYes, in nude mice. Yes, in soft agar.

Specifications

Complete Growth MediumMEM (PM150410)+10% FBS (164210-500)+1% P/S (PB180120)
Subcultivation Ratio1:2-1:4
Medium Renewalevery 2 to 3 days
CryopreservationFreeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture ConditionsAtmosphere: Air, 95%; CO2, 5% Temperature: 37℃
TumorigenicYes
Receptor Expressionurokinase receptor(u-PAR)
DurationIt can be used as the control cell line for investigating the effects of p53 and gadd45 on cellular parameters.

Misc Information

SubculturingRemove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
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