tcel189 Raji

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Product Description

These cells are EBNA positive. The cells are partially resistant to poliovirus and vesicular stomatitis viruses. ATCC confirmed this line tested positive for the presence of Epstein Barr virus(EBV) viral DNA sequences via PCR.

Information

Applicationtransfection
TissueHuman lymphoblast
Morphologylymphoblast
Growth Propertiessuspension

Specifications

Complete Growth MediumRPMI-1640 (PM150110)+10% FBS (164210-500)+1% P/S (PB180120)
Subcultivation Ratio1:2-1:4
Medium Renewalevery 2 to 3 days
CryopreservationFreeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture ConditionsAtmosphere: Air, 95%; CO2, 5% Temperature: 37℃
Durationtransfection

Misc Information

SubculturingCultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2×10^5 viable cells/ml. Maintain cell density between 1×10^5 and 1×10^6 viable cells/ml.
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