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Cell Line
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tcel175
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AVAILABLE SIZES
1×10⁶cells/t25culturebottle
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
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Western blot (WB)
Immunohistochemistry (IHC)
Immunofluorescence (IF)
Immunocytochemistry (ICC)
Immunoprecipitation (IP)
Co-Immunoprecipitation (CoIP)
Chromatin Immunoprecipitation (ChIP)
RNA Binding Protein Immunoprecipitation (RIP)
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UG
CELLS
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Product Description
No product descriptions available. Please refer to the product information & specifications for details.
Information
Application
This cell line is a suitable transfection host. This line was originally developed for use as a source of X chromosomes in a study of X inactivation, and was subsequently found to be an excellent cell culture model for the kidney proximal tubule epitheliu
Tissue
Opossum kidney, cortex/proximal tubule
Morphology
epithelial
Growth Properties
adherent
Specifications
Complete Growth Medium
MEM (PM150410)+10% FBS (164210-500)+1% P/S (PB180120)
Subcultivation Ratio
1:2-1:4
Medium Renewal
every 2 to 3 days
Cryopreservation
Freeze medium: 60% Basal medium＋30% FBS＋10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture Conditions
Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
Duration
This cell line is a suitable transfection host. This line was originally developed for use as a source of X chromosomes in a study of X inactivation, and was subsequently found to be an excellent cell culture model for the kidney proximal tubule epitheliu
Misc Information
Subculturing
Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
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