tcel132 KG-1

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Product Description

KG-1 myeloblasts can differentiate into macrophages in the presence of phorbol esters.They show a good response to colony stimulating factor(CSF).The line is EBNA negative(EBNA-).

Information

Applicationtransfection
TissueHuman bone marrow
Morphologymyeloblast
Growth Propertiessuspension

Specifications

Complete Growth MediumIMDM (PM150510)+20% FBS (164210-500)+1% P/S (PB180120)
Subcultivation Ratio1:2-1:4
Medium Renewalevery 2 to 3 days
CryopreservationFreeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture ConditionsAtmosphere: Air, 95%; CO2, 5% Temperature: 37℃
Antigen ExpressionHLA A30, A31, B35, Cw4
Gene ExpressionHLA DR
Durationtransfection

Misc Information

SubculturingCultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at the subcultivation ratio of 1:2-1:3.
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