Product Description
Clone E6-1 cells produce large amounts of IL-2 after stimulation with phorbol esters and either lectins or monoclonal antibodies against the T3 antigen(both types of stimulants are needed to induce IL-2 production.
Information
Applicationtransfection
TissueHuman peripheral blood
Morphologylymphoblast
Growth Propertiessuspension
Specifications
Complete Growth MediumRPMI-1640 (PM150110)+10% FBS (164210-500)+1% P/S (PB180120)
Subcultivation Ratio1:2-1:4
Medium Renewalevery 2 to 3 days
CryopreservationFreeze medium: 60% Basal medium＋30% FBS＋10% DMSO
Storage temperature: Liquid nitrogen vapor phase
Culture ConditionsAtmosphere: Air, 95%; CO2, 5%
Temperature: 37℃
Receptor ExpressionReceptor expression: T cell antigen receptor, expressed
Antigen ExpressionCD3; Homo sapiens, expressed
Gene Expressioninterleukin-2(interleukin 2, IL-2), CD3; Homo sapiens, expressed
Durationtransfection
Misc Information
SubculturingCultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at the subcultivation ratio of 1:2-1:4.