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Cell Line
HUV-EC-C [HUVEC]
tcel122
HUV-EC-C [HUVEC]
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AVAILABLE SIZES
1x10^6 Cells/Vial
$
0.00
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
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Western blot (WB)
Immunohistochemistry (IHC)
Immunofluorescence (IF)
Immunocytochemistry (ICC)
Immunoprecipitation (IP)
Co-Immunoprecipitation (CoIP)
Chromatin Immunoprecipitation (ChIP)
RNA Binding Protein Immunoprecipitation (RIP)
Sample
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UG
CELLS
Treatment
Temperature
Exposure
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Fixative
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Acetone
Formaldehyde
Methanol
Paraformaldehyde
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Heat mediated
Enzymatic
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Product Description
Endothelial Cell Growth Supplement(ECGS) and unidentified factors from bovine pituitary, hypothalamus or whole brain extracts are mitogenic for this line. The cells have a life expectancy of 50 to 60 population doublings.
Information
Application
transfection
Tissue
Human umbilical vein/vascular endothelium
Morphology
endothelial
Growth Properties
adherent
Effects
Yes, the cells did form colonies in semisolid medium
No, the cells were not tumorigenic in immunosuppressed mice.
Specifications
Complete Growth Medium
90% Medium 199 with 25U/ml heparin, 30ug/ml endothelial cell growth supplement(ECGS) adjusted to contain 1.5 g/L sodium bicarbonate +10% fetal bovine serum and 100U/ml Penicillin/Streptomycin .
Subcultivation Ratio
1:2-1:4
Medium Renewal
every 2 to 3 days
Cryopreservation
Freeze medium: 93% culture medium(with 20% FBS)+7%DMSO Storage temperature: Liquid nitrogen vapor phase
Culture Conditions
Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
Tumorigenic
No
Gene Expression
factor Ⅷ
Duration
transfection
Misc Information
Notes
Culture flask or culture dish should be treated with 1％ gelatin before used
Subculturing
Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
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