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Product Description
HL-60 cells spontaneously differentiate and differentiation can be stimulated by butyrate, Hypoxanthine, phorbol myristic acid(PMA, TPA), dimethylsulfoxide(DMSO, 1% to 1.5%), actinomycin D, and retinoic acid.The cells exhibit phagocytic activity and responsiveness to chemotactic stimuli.The line is positive for myc oncogene expression.
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Information
Applicationtransfection
TissueHuman peripheral blood
Morphologymyeloblast
Growth Propertiessuspension
EffectsYes, in nude mice(subcutaneous myeloid tumors). Yes, in semi-solid media.
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Specifications
Complete Growth MediumIMDM (PM150510)+20% FBS (164210-500)+1% P/S (PB180120)
Subcultivation Ratio1:2-1:4
Medium Renewalevery 2 to 3 days
CryopreservationFreeze medium: 60% Basal medium+30% FBS+10% DMSO
Storage temperature: Liquid nitrogen vapor phase
Culture ConditionsAtmosphere: Air, 95%; CO2, 5%
Temperature: 37℃
TumorigenicYes
Receptor Expressioncomplement, expressed; Fc, expressed
Gene Expressiontumor necrosis factor(TNF), also known as tumor necrosis factor alpha(TNF-alpha, TNF alpha), after stimulation with phorbol myristic acid, myc+
Durationtransfection
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Misc Information
SubculturingCultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2×10^5 viable cells/ml. Maintain cell density between 1×10^5 and 1×10^6 viable cells/ml.