Product Description
HL-60 cells spontaneously differentiate and differentiation can be stimulated by butyrate, Hypoxanthine, phorbol myristic acid(PMA, TPA), dimethylsulfoxide(DMSO, 1% to 1.5%), actinomycin D, and retinoic acid.The cells exhibit phagocytic activity and responsiveness to chemotactic stimuli.The line is positive for myc oncogene expression.
Information
Applicationtransfection
TissueHuman peripheral blood
Morphologymyeloblast
Growth Propertiessuspension
EffectsYes, in nude mice(subcutaneous myeloid tumors). Yes, in semi-solid media.
Specifications
Complete Growth MediumIMDM (PM150510)+20% FBS (164210-500)+1% P/S (PB180120)
Subcultivation Ratio1:2-1:4
Medium Renewalevery 2 to 3 days
CryopreservationFreeze medium: 60% Basal medium＋30% FBS＋10% DMSO
Storage temperature: Liquid nitrogen vapor phase
Culture ConditionsAtmosphere: Air, 95%; CO2, 5%
Temperature: 37℃
TumorigenicYes
Receptor Expressioncomplement, expressed; Fc, expressed
Gene Expressiontumor necrosis factor(TNF), also known as tumor necrosis factor alpha(TNF-alpha, TNF alpha), after stimulation with phorbol myristic acid, myc＋
Durationtransfection
Misc Information
SubculturingCultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2×10^5 viable cells/ml. Maintain cell density between 1×10^5 and 1×10^6 viable cells/ml.