Product Description
The cells express 3-hydroxy-3-methylglutaryl-CoA reductase and hepatic triglyceride lipase activities.The cells demonstrate decreased expression of apoA-I mRNA and increased expression of catalase mRNA in response to gramoxone(oxidative stress).There is no evidence of a Hepatitis B virus genome in this cell line.
Information
ApplicationThese cells are suitable as a transfection host.
TissueHuman liver
Morphologyepithelial
Growth Propertiesadherent
EffectsNo, in immunosuppressed mice
Yes, in semisolid medium.
Specifications
Complete Growth MediumMEM (PM150410)+10% FBS (164210-500)+1% P/S (PB180120)
Subcultivation Ratio1:2-1:4
Medium Renewalevery 2 to 3 days
CryopreservationFreeze medium: 60% Basal medium＋30% FBS＋10% DMSO
Storage temperature: Liquid nitrogen vapor phase
Culture ConditionsAtmosphere: Air, 95%; CO2, 5%
Temperature: 37℃
TumorigenicNo
Receptor ExpressionInsulin; Insulin-like growth factorⅡ(IGFⅡ)
Gene Expressionalpha-fetoprotein(alpha fetoprotein); albumin; alpha2 macroglobulin(alpha-2-macroglobulin); alpha1 antitrypsin(alpha-1-antitrypsin); Transferrin; alpha1 antichymotrypsin;(alpha-1-antichymotrypsin); haptoglobin; ceruloplasmin; plasminogen;, complement(C4);
DurationThese cells are suitable as a transfection host.
Misc Information
SubculturingRemove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor.
Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.