Products
PRIMARY ANTIBODY
SECONDARY ANTIBODY
PROTEIN & PEPTIDES
BIOCHEMICALS
ASSAY & ELISA KITS
LOADING CONTROL
OTHER REAGENTS
SERUMS
CELL LINE
Contact Us
About Us
Login
Register
|
Forgot Password
Sign In
Forgot your Password?
If you've forgotten your password, enter your e-mail address below and we'll send you instructions on how to securely change your password.
Back
Continue
0
Shopping Cart
Products
Contact Us
About Us
Shopping Cart
Loading....
Login Account
Sign In
Register
Register
|
Forgot Password
Sign In
Forgot your Password?
If you've forgotten your password, enter your e-mail address below and we'll send you instructions on how to securely change your password.
Back
Continue
0
Shopping Cart
Home
Cell Line
F9
tcel82
F9
Order Now
AVAILABLE SIZES
1×10⁶cells/t25culturebottle
$
0.00
Add to Cart
ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
Data sheet
Download PDF Datasheet
Technical Inquries
Submit Review
Submit Review
Please choose your application, enter the information requested below. * Marked fields are mandatory.
Full Name
Email
Contact Number
Application
Please Select..
Western blot (WB)
Immunohistochemistry (IHC)
Immunofluorescence (IF)
Immunocytochemistry (ICC)
Immunoprecipitation (IP)
Co-Immunoprecipitation (CoIP)
Chromatin Immunoprecipitation (ChIP)
RNA Binding Protein Immunoprecipitation (RIP)
Sample
Loading Amount
Loading Type
UG
CELLS
Treatment
Temperature
Exposure
Observe Band
Fixative
Select One
Acetone
Formaldehyde
Methanol
Paraformaldehyde
Other
Antigen Retrieval
Select One
Heat mediated
Enzymatic
Other
None
Positive Control
Negative Control
Dilution *
1:
Incubation Time *
Select Image *
Choose a file...
Rating *
Remarks *
Submit Review
Product Description
F9 cells can be stimulated to differentiate into parietal endoderm in the presence of retinoic acid and dibutyryl cyclic AMP(cAMP).Differentiating cells synthesize plasminogen activator, laminin and type IV collagen.cAMP is active only on cells that have been treated with retinoic acid.The cells maintain three copies of the beta 1 integrin gene.Tested and found negative for ectromelia virus(mousepox).
Information
Application
transfection host
Tissue
Mouse testis
Morphology
epithelial
Growth Properties
adherent
Specifications
Complete Growth Medium
DMEM (PM150210)+10% FBS (164210-500)+1% P/S (PB180120)
Subcultivation Ratio
1:2-1:4
Medium Renewal
every 2 to 3 days
Cryopreservation
Freeze medium: 60% Basal medium＋30% FBS＋10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture Conditions
Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
Gene Expression
plasminogen activator; laminin; type IV collagen
Duration
transfection host
Misc Information
Subculturing
Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
related data
Get valuable resources and offers directly to your email.
REGISTER NOW