Product Description
The cells exhibit ion transport activities consistent with cystic fibrosis and express the product of the CF gene(cystic fibrosis transmembrane regulator, CFTR).CFPAC-1 cells show no effect of cAMP agonists, adenyl cyclase stimulators or phosphodiesterase inhibitors on Cl- flux, but do respond to Ca＋ionophores with increase Cl- efflux.The cells have the most common form of the CF mutation, deletion of three nucleotides resulting in the absence of phenylalanine at position 508.CFPAC-1 cells have epithelial morphology and polarization with apical microvilli, tight junctions and gap junctions.
Information
Applicationtransfection host
TissueHuman pancreas
derived from metastatic: liver
Morphologyepithelial
Growth Propertiesadherent
EffectsYes, in nude mice.
Specifications
Complete Growth MediumIMDM (PM150510)+10% FBS (164210-500)+1% P/S (PB180120)
Subcultivation Ratio1:2-1:4
Medium Renewalevery 2 to 3 days
CryopreservationFreeze medium: 60% Basal medium＋30% FBS＋10% DMSO
Storage temperature: Liquid nitrogen vapor phase
Culture ConditionsAtmosphere: Air, 95%; CO2, 5%
Temperature: 37℃
TumorigenicYes
Antigen ExpressionCA19-9 antigen, 12000 units/mL, epithelial keratins
Gene Expressioncarcinoembryonic antigen(CEA), 9ng/mL; pancreatic oncofetal antigen(POA), 28ng/mL; adenocarcinoma associated antigen(ACAA), 5000ng/mL; CA 19-9 antigen, 12000 units/mL; epithelial keratins
Durationtransfection host
Misc Information
SubculturingRemove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor.
Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.