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Cell Line
6T-CEM
tcel8
6T-CEM
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AVAILABLE SIZES
1×10⁶cells/t25culturebottle
$
0.00
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
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Western blot (WB)
Immunohistochemistry (IHC)
Immunofluorescence (IF)
Immunocytochemistry (ICC)
Immunoprecipitation (IP)
Co-Immunoprecipitation (CoIP)
Chromatin Immunoprecipitation (ChIP)
RNA Binding Protein Immunoprecipitation (RIP)
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UG
CELLS
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Product Description
This is a 6-thioguanine resistant variant of CCRF-CEM.It is deficient in Hypoxanthine phosphoribosyl transferase(HPRT, HGPRT) and secretes high titers of an immunosuppressive factor.This line may be used as a fusion partner for making human T cell hybridomas.suspensionimmunosuppressive factorCultures can be maintained by addition or replacement of fresh medium. Start cultures at 2×10 exp5 cells/ml and maintain between 1×10 exp5 and 1×10 exp6 cells/ml.Handle as potentially biohazardous material under at least Biosafety Level 2 containment.
Information
Tissue
Human peripheral blood
Morphology
lymphoblast
Growth Properties
suspension
Specifications
Complete Growth Medium
RPMI-1640 (PM150110)+10% FBS (164210-500)+1% P/S (PB180120)
Subcultivation Ratio
1:2-1:4
Medium Renewal
2 to 3 times per week
Cryopreservation
Freeze medium: 60% Basal medium＋30% FBS＋10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture Conditions
Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
Misc Information
Subculturing
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at the subcultivation ratio of 1:2-1:4.
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