tcel2 5637

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Product Description
The 5637 cell line has been established from the primary bladder carcinoma(grade Ⅱ) ofa patient by Dr. G. Cannon in 1974.

Information
Applicationtransfection host
TissueHuman urinary bladder
Morphologyepithelial
Growth Propertiesadherent
EffectsYes, within 21 days at 100% frequency(5/5) in nude mice inoculated subcutaneously with 10^7 cells.

Specifications
Complete Growth MediumRPMI-1640 (PM150110)+10% FBS (164210-500)+1% P/S (PB180120)
Subcultivation Ratio1:3-1:6
Medium Renewal2 to 3 times per week
CryopreservationFreeze medium: 60% Basal medium＋30% FBS＋10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture ConditionsAtmosphere: Air, 95%; CO2, 5% Temperature: 37℃
TumorigenicYes
Durationtransfection host

Misc Information
SubculturingRemove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
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