tcba13739 Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)

Immunofluorescence analysis of U-2 OS cells, using LMNB1 antibody as the primary antibody at dilution of 1:100. The cells were incubated with the primary antibody overnight at 4°C. Secondary antibody: Alexa Fluor 488-conjugated AffiniPure Goat Anti-RabbitIgG(H+L) (tcba13739) at 1:100 dilution. Blue: DAPI for nuclear staining.Immunofluorescence analysis of U-2 OS cells, using LMNB1 antibody as the primary antibody at dilution of 1:100. The cells were incubated with the primary antibody overnight at 4°C. Secondary antibody: Alexa Fluor 488-conjugated AffiniPure Goat Anti-RabbitIgG(H+L) (tcba13739) at 1:200 dilution. Blue: DAPI for nuclear staining.Immunofluorescence analysis of U-2 OS cells, using LMNB1 antibody as the primary antibody at dilution of 1:100. The cells were incubated with the primary antibody overnight at 4°C. Secondary antibody: Alexa Fluor 488-conjugated AffiniPure Goat Anti-RabbitIgG(H+L) (tcba13739) at 1:400 dilution. Blue: DAPI for nuclear staining.Immunofluorescence analysis of U-2 OS cells, using LMNB1 antibody as the primary antibody at dilution of 1:100. The cells were incubated with the primary antibody overnight at 4°C. Secondary antibody: Alexa Fluor 488-conjugated AffiniPure Goat Anti-RabbitIgG(H+L) (tcba13739) at 1:800 dilution. Blue: DAPI for nuclear staining.Immunofluorescence analysis of U-2 OS cells, using LMNB1 antibody as the primary antibody at dilution of 1:100. The cells were incubated with the primary antibody overnight at 4°C. Secondary antibody: Alexa Fluor 488-conjugated AffiniPure Goat Anti-RabbitIgG(H+L) (tcba13739) at 1:1000 dilution. Blue: DAPI for nuclear staining.
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Product Description
Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)

Information
Host SpeciesGoat
Immunogen / Amino acidsRabbit IgG
ConjugationAlexa Fluor 488
IsotypeIgG

Specifications
Storage BufferStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Tested ApplicationImmunofluorescence Flow Cytometry
Recommended DilutionImmunofluorescence 1:100 - 1:800
Flow Cytometry 1:100 - 1:800

Misc Information
PurificationAffinity purification
Alexa Fluor is a registered trademark of Life Technologies Corporation
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