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Primary Antibody
Human IgE (SUS-11) Full Range
tcnbp113
Human IgE (SUS-11) Full Range
size exclusion chromatography
Target: rec. human high affinity IgE receptor;. (Surface Plasmon Resonance; Biacore™):
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1000 μg
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
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Western blot (WB)
Immunohistochemistry (IHC)
Immunofluorescence (IF)
Immunocytochemistry (ICC)
Immunoprecipitation (IP)
Co-Immunoprecipitation (CoIP)
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RNA Binding Protein Immunoprecipitation (RIP)
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Product Description
Recombinant human IgE to be used as reference for IgE concentration measurements in biological samples (ELISA; FACS). IgE (SUS-11) recognizes the human high affinity IgE receptor (FcRI), the human low affinity IgE receptor (CD23) and does not interact with the corresponding IgE receptors of other species. It can be used as functional reagent in mast cell / basophil cellular assays, e.g. for controlled sensitization of such cells and subsequent cross-linking with either anti-human IgE mAbs like mAb LE27 or a specific recombinant cross-linking agent.
Information
Application
ELISA, FACS Western blot, functional cellular assays (mast cell / basophil activation).
Species Reactivity
Specific allergen unknown.
Cross reactivity
> 95 %, compared to calibrator (Internal Standard IgE (SUS-11), tested by SPR (Biacore™) binding to immobilized recombinant human high affinity IgE receptor
Clones
SUS11-NBS01
Isotype
Human IgE
Specifications
Storage Buffer
Phosphate buffered saline (PBS); pH = 7.2; sterile filtered
Concentration
1mg/ml
Source
Human B cells hybridoma from healthy donors (CD40 cell culture system)
Purity / Grade
> 95%, tested by analytical Gel Permeation Chromatography
Misc Information
Storage Instruction
Store undiluted at 4°C. For long term storage, aliquot and store at -20°C or -80°C. Avoid repeated freeze-thawing. Handle under sterile conditions, the mAb is formulated in sterile buffer solution and does not contain sodium azide as preservative. The product may be shipped on ice pack or dry ice.
Purification
Affinity - purified from cell culture supernatant by Immunoaffinity Chromatography on CH-Sepharose 4B – mouse anti-human IgE mAb
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