tcna10436 GLO1 Antibody / Glyoxalase I

Western blot testing of human 1) HeLa, 2) placenta, 3) MCF7, 4) COLO320, 5) 22RV1, 6) HepG2, 7) A431 and 8) U937 lysate with GLO1 antibody at 0.5ug/ml. Predicted molecular weight ~21 kDa.Western blot testing of rat 1) brain, 2) testis, 3) spleen, 4) thymus and mouse 5) brain, 6) testis, 7) thymus and 8) liver lysate with GLO1 antibody at 0.5ug/ml. Predicted molecular weight ~21 kDa.IHC testing of FFPE human lung cancer tissue with GLO1 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.IHC testing of FFPE human breast cancer tissue with GLO1 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.IHC testing of FFPE rat small intestine tissue with GLO1 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.IHC testing of FFPE rat spleen tissue with GLO1 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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Product Description
Lactoylglutathione lyase in humans is encoded by the GLO1 gene. The enzyme encoded by this gene is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutatione. Glyoxalase I is linked to HLA and is localized to 6p21.3-p21.1, between HLA and the centromere.

Information
ApplicationWB, IHC-P, ELISA
Species ReactivityHuman, Mouse, Rat
Host SpeciesRabbit
Immunogen / Amino acidsA recombinant human protein corresponding to amino acids A2-M184 was used as the immunogen for the GLO1 antibody.
ConjugationAntigen affinity purified
ClonalityPolyclonal
IsotypeRabbit IgG

Specifications
FormLyophilized powder
Storage BufferLyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Recommended DilutionWestern Blot: 0.5-1ug/ml
IHC (FFPE): 1-2ug/ml
Direct ELISA: 0.1-0.5ug/mlOptimal dilution of the GLO1 antibody should be determined by the researcher.

Misc Information
Storage InstructionAfter reconstitution, the GLO1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
SwissProtQ04760
ReferencesAntigen affinity purified
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