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Primary Antibody
GADD34 Antibody
tcna7651
GADD34 Antibody
Western blot testing of 1) HEPG2 cell lysate and 2) KLY negative control cell lysate with GADD34 antibody at 0.1ug/ml. Predicted molecular weight ~73 kDa.
IHC staining of FFPE human liver with GADD34 antibody at 2ug/ml. HIER: steamed with pH6 citrate buffer, HRP-staining.
ICC/IF testing of fixed and permeabilized human HepG2 cells with GADD34 antibody (green) at 10ug/ml and DAPI nuclear stain (blue).
ICC/IF testing of fixed and permeabilized human HeLa cells with GADD34 antibody (green) at 10ug/ml and DAPI nuclear stain (blue).
FACS testing of fixed and permeabilized human HepG2 cells with GADD34 antibody (blue) at 10ug/10^6 cells and naive goat Ig (black).
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AVAILABLE SIZES
100ug
$
498.00
Add to Cart
ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
Data sheet
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Application
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Western blot (WB)
Immunohistochemistry (IHC)
Immunofluorescence (IF)
Immunocytochemistry (ICC)
Immunoprecipitation (IP)
Co-Immunoprecipitation (CoIP)
Chromatin Immunoprecipitation (ChIP)
RNA Binding Protein Immunoprecipitation (RIP)
Sample
Loading Amount
Loading Type
UG
CELLS
Treatment
Temperature
Exposure
Observe Band
Fixative
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Acetone
Formaldehyde
Methanol
Paraformaldehyde
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Antigen Retrieval
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Heat mediated
Enzymatic
Other
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Positive Control
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Product Description
Additional name(s) for this target protein: Growth arrest and DNA-damage-inducible 34; PPP1R15A
Information
Application
WB, IHC, ICC/IF, FACS, ELISA (peptide)
Species Reactivity
Human
Host Species
Goat
Immunogen / Amino acids
Amino acids AAALDLSGRRG were used as the immunogen for this GADD34 antibody.
Conjugation
Antigen affinity purified
Clonality
Polyclonal
Isotype
Goat Ig
Specifications
Form
Liquid
Storage Buffer
0.5 mg/ml in 1X TBS, pH7.3, with 0.5% BSA (US sourced) and 0.02% sodium azide
Recommended Dilution
Western blot: 0.1-0.3ug/ml
Immunohistochemistry (FFPE): 2-4ug/ml
ICC/IF: 10ug/ml
FACS: 10ug/10^6 cells
ELISA (peptide) LOD: 1:128000Optimal dilution of the GADD34 antibody should be determined by the researcher.
1. Not suitable for ICC/IF testing
Misc Information
Storage Instruction
Aliquot and store the GADD34 antibody at -20oC.
Gene ID
23645
(human);
References
Antigen affinity purified
related data
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