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Primary Antibody
TCP1 delta Antibody / CCT4
tcna7081
TCP1 delta Antibody / CCT4
Western blot testing of 1) rat brain, 2) mouse brain and 3) human HeLa lysate with TCP1 delta antibody. Expected/observed molecular weight ~58 kDa.
IHC testing of FFPE human breast cancer tissue with TCP1 delta antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to testing.
IHC testing of FFPE mouse kidney with TCP1 delta antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to testing.
IHC testing of FFPE rat brain with TCP1 delta antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to testing.
IHC testing of frozen mouse brain tissue with TCP1 antibody.
IHC testing of frozen rat brain tissue with TCP1 antibody.
ICC testing of FFPE human SHG-44 cells with TCP1 delta antibody.
ICC testing of FFPE mouse Neuro-2a cells with TCP1 delta antibody.
ICC testing of FFPE rat NRK cells with TCP1 delta antibody.
Flow cytometry testing of human U-251 MG cells with TCP1 delta antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TCP1 delta antibody.
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AVAILABLE SIZES
100ug
$
498.00
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
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Western blot (WB)
Immunohistochemistry (IHC)
Immunofluorescence (IF)
Immunocytochemistry (ICC)
Immunoprecipitation (IP)
Co-Immunoprecipitation (CoIP)
Chromatin Immunoprecipitation (ChIP)
RNA Binding Protein Immunoprecipitation (RIP)
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Product Description
T-complex protein 1 subunit delta is a protein that in humans is encoded by the CCT4 gene. This gene is mapped to 2p15. The chaperonin containing TCP1 complex (CCT), also called the TCP1 ring complex, consists of 2 back-to-back rings, each containing 8 unique but homologous subunits, such as CCT4. CCT assists the folding of newly translated polypeptide substrates through multiple rounds of ATP-driven release and rebinding of partially folded intermediate forms. Substrates of CCT include the cytoskeletal proteins actin and tubulin, as well as alpha-transducin.
Information
Application
WB, IHC-P, IHC-F, ICC, FACS
Species Reactivity
Human, Mouse, Rat
Host Species
Rabbit
Immunogen / Amino acids
Amino acids R452-R539 of the human protein were used as the immunogen for the TCP1 delta antibody.
Conjugation
Antigen affinity purified
Clonality
Polyclonal
Isotype
Rabbit IgG
Specifications
Form
Lyophilized powder
Storage Buffer
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Recommended Dilution
Western blot: 0.1-0.5ug/ml
IHC (FFPE): 0.5-1ug/ml
IHC (Frozen): 0.5-1ug/ml
ICC (FFPE): 0.5-1ug/ml
FACS: 1-3ug/10^6 cellsOptimal dilution of the TCP1 delta antibody should be determined by the researcher.
Misc Information
Storage Instruction
After reconstitution, the TCP1 delta antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
SwissProt
P50991
References
Antigen affinity purified
related data
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