tcna6680 Lamin B1 Antibody

Western blot testing of 1) human U87, 2) rat PC-12 and 3) mouse NIH3T3 lysate with LMNB1 antibody. Predicted molecular weight ~66 kDa.IHC testing of FFPE human breast cancer tissue with LMNB1 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.IHC testing of FFPE mouse intestine with LMNB1 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.IHC testing of FFPE rat intestine with LMNB1 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.IHC testing of frozen human placental tissue with LMNB1 antibody.IHC testing of frozen mouse small intestine tissue with LMNB1 antibody.IHC testing of frozen rat small intestine tissue with LMNB1 antibody.ICC testing of FFPE human SW480 cells with LMNB1 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.ICC testing of FFPE human SMMC-7721 cells with LMNB1 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.ICC testing of FFPE human A549 cells with LMNB1 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
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Product Description
Lamin B1 is a protein that in humans is encoded by the LMNB1 gene. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1.

Information
ApplicationWB, IHC-P, IHC-F, ICC
Species ReactivityHuman, Mouse, Rat
Host SpeciesRabbit
Immunogen / Amino acidsAmino acids 266-583 of human Lamin B1 were used as the immunogen for the LMNB1 antibody.
ConjugationAntigen affinity purified
ClonalityPolyclonal
IsotypeRabbit IgG

Specifications
FormLyophilized powder
Storage Buffer0.5mg/ml if reconstituted with 0.2ml sterile DI water
Recommended DilutionWestern blot: 0.1-0.5ug/ml
IHC (Paraffin): 0.5-1ug/ml
IHC (Frozen): 0.5-1ug/ml
ICC (Paraffin): 0.5-1ug/mlOptimal dilution of the LMNB1 antibody should be determined by the researcher.

Misc Information
Storage InstructionAfter reconstitution, the LMNB1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
SwissProtP20700
ReferencesAntigen affinity purified
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