Product Description
Collagen type 1 alpha 1, also known as COL1A1, is a human gene that encodes the major component of type 1 collagen, the fibrillar collagen found in most connective tissues, including cartilage. This gene is mapped to 17q21.33. This gene encodes the pro-alpha1 chains of type 1 collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Collagen type 1 is fibril-forming and found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis.
Information
ApplicationWB, IHC-P, IHC-F, ICC
Species ReactivityHuman, Mouse, Rat
Host SpeciesRabbit
Immunogen / Amino acidsAn amino acid sequence from the C-terminus of mouse COL1A1 (QPPQEKSQDGGRYYRA) was used as the immunogen for this Collagen type 1 antibody (100% rat homology).
ConjugationAntigen affinity purified
ClonalityPolyclonal
IsotypeRabbit IgG
Specifications
FormLyophilized powder;Lyophilized from 1X PBS with 2% Trehalose
Storage Buffer0.5mg/ml if reconstituted with 0.2ml sterile DI water
Tested ApplicationWB, IHC, ELISA
Recommended DilutionWestern blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml
Immunohistochemistry (Frozen) : 1-2ug/ml
Immunocytochemistry (FFPE) : 1-2ug/ml
Immunofluorescence (FFPE) : 2ug/ml
Purity / GradeAntigen affinity
Misc Information
Storage InstructionAfter reconstitution, the Collagen type 1 antibody can be stored for up to one month at 4ºC. For long-term, aliquot and store at -20ºC. Avoid repeated freezing and thawing.
ReferencesAntigen affinity purified
Cellular LocationCytoplasmic