tcna4996 Beta-Actin Antibody

Western blot testing of beta-Actin antibody and rat tissue: ovary (1), testis (2), heart (3), brain (4); and human cell lines, lanes 5: A453; 6: HeLa; 7: SMMC-7721; 8: HT1080; 9: SW620 cell lysateWestern blot testing of beta-Actin antibody and rat tissues: liver (1), spleen (2), brain (3), kidney (4) and human cell lines, lane 5: HeLa; 6: SMMC-7721; 7: SGC cell lysateIHC testing of FFPE human lung cancer tissue with beta-Actin antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to testing.IHC testing of FFPE human intestinal cancer tissue with beta-Actin antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to testing.IHC testing of FFPE human breast cancer tissue with beta-Actin antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to testing.IHC testing of FFPE mouse intestine with beta-Actin antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to testing.IHC testing of FFPE rat intestine with beta-Actin antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to testing.
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Product Description
The primary site of action of cytochalasin B on cell motility processes is beta-actin. Habets et al.(1992) generated hybrids that harbor only specific regions of human chromosome 7 and assigned the ACTB locus to 7p15-p12. ACTB and the other assigned beta-actin-related sequences are dispersed over at least four different chromosomes including one locus assigned to the X chromosome. A mutation of the protein that alters depolymerization dynamics is associated with autosomal dominant developmental malformations, deafness, and dystonia.

Information
ApplicationWB, IHC-P
Species ReactivityHuman, mouse, rat
Host SpeciesMouse
Immunogen / Amino acidsAmino acids DDDIAALVIDNGSGK were used as the immunogen for this beta-Actin antibody.
ConjugationAscites
ClonalityMonoclonal
ClonesAC-15
IsotypeMouse IgG1

Specifications
FormLiquid
Storage Buffer0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS
Recommended DilutionWestern blot: 0.25-0.5ug/ml
IHC (Paraffin): 0.5-1ug/mlThe stated application concentrations are suggested starting points. Titration of the beta-Actin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Misc Information
Storage InstructionAfter reconstitution, the beta-Actin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Gene ID60 (human);
ReferencesAscites
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