tcna4773 Recombinant Acetyl Lysine Antibody

ICC staining of human HeLa cells using recombinant Acetyl Lysine antibody (red). Actin filaments was labeled with fluorescein phalloidin (green), and nuclei stained with DAPI (blue).Western blot of human HeLa cells non-treated or treated with Trichostatin A (TSA) with recombinant Acetyl Lysine antibody at 1:2000.IP of human A431 cell lysate with recombinant Acetyl Lysine antibody at 1:500 and probed with anti-PTEN mouse mAb.The recombinant Acetyl Lysine antibody recognizes acetylated lysine in peptides with different sequences. It does not react with non-acetylated lysine.The recombinant Acetyl Lysine antibody recognizes acetylated lysine in peptides with different sequences. It does not react with non-acetylated lysine.
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Product Description
This recombinant Acetyl Lysine antibody reacts to lysine-acetylated proteins. No cross reactivity with non-acetylated lysine, and lysine with other modification.

Information
ApplicationWB, IP, ICC, IHC, FACS, ELISA
Species ReactivityAll Species
Host SpeciesRabbit
Immunogen / Amino acidsAcetyllysine-BSA was used as the immunogen for this recombinant Acetyl Lysine antibody.
ConjugationPurified
ClonalityRecombinant Rabbit Monoclonal
ClonesRM101
IsotypeRabbit IgG

Specifications
FormLiquid
Storage Buffer1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Recommended DilutionWestern Blot: 1:500 -1:2000 dilution
Immunoprecipitation: 1:100-1:500 dilution
Chromatin IP (ChIP): 1:100-1:500 dilution
Immunocytochemistry: 1:100-1:500 dilution
Immunohistochemistry: 1:100-1:500 dilution (1)The stated application concentrations are suggested starting points. Titration of the recombinant Acetyl Lysine antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Misc Information
Storage InstructionStore the recombinant Acetyl Lysine antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).
Gene IDN/A (human);
ReferencesPurified
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