tcna293pe Nuclear Marker Antibody PE Conjugate

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Product Description

This Nuclear marker antibody is part of a panel of reagents which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. Clone 235-1 antibody specifically detects an antigen associated with the nuclei in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed or frozen tissue sections. The human nuclear antigen antibody can also be used with paraformaldehyde fixed frozen tissue or cell preparations.

Information

ApplicationFACS, IF
Species ReactivityHuman and Primates. Does not react with mouse, rat and chicken. Other species not known.
Host SpeciesMouse
Immunogen / Amino acidsNuclei of human myeloid leukemia biopsy cells were used as the immunogen for this Nuclear marker antibody.
ConjugationPE
ClonalityMonoclonal
Clones235-1
IsotypeMouse IgG1, kappa

Specifications

FormLiquid
Storage Buffer500 ul at 0.1 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Concentration0.1 mg/ml
Recommended DilutionFlow Cytometry : 5ul/test/million cells or 5ul/test/100ul of whole blood
Immunofluorescence : 1:50-1:100 for 30 minutes at RTThe concentration stated for each application is a general starting point. Variations in protocols
secondaries and substrates may require the Nuclear marker antibody to be titered up or down for optimal performance.

Misc Information

Storage InstructionStore the human Nuclear antigen antibody at 2-8°C. Conjugate is light sensitive, store in the dark.
SwissProtNot Known
Gene IDUnknown (human);
ReferencesProtein G affinity chromatography
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