Products
PRIMARY ANTIBODY
SECONDARY ANTIBODY
PROTEIN & PEPTIDES
BIOCHEMICALS
ASSAY & ELISA KITS
LOADING CONTROL
OTHER REAGENTS
SERUMS
CELL LINE
Contact Us
About Us
Login
Register
|
Forgot Password
Sign In
Forgot your Password?
If you've forgotten your password, enter your e-mail address below and we'll send you instructions on how to securely change your password.
Back
Continue
0
Shopping Cart
Products
Contact Us
About Us
Shopping Cart
Loading....
Login Account
Sign In
Register
Register
|
Forgot Password
Sign In
Forgot your Password?
If you've forgotten your password, enter your e-mail address below and we'll send you instructions on how to securely change your password.
Back
Continue
0
Shopping Cart
Home
Primary Antibody
Nuclear Marker Antibody PE Conjugate
tcna293pe
Nuclear Marker Antibody PE Conjugate
FACS testing of MCF-7 cells: Black=cells alone; Green=isotype control; Red= Nuclear marker antibody
FACS testing of HeLa cells: Black=cells alone; Green=isotype control; Red= Nuclear marker antibody
SDS-PAGE Analysis of Purified, BSA-Free, Unlabeled Human Nuclear Antigen Antibody (clone 235-1). Confirmation of Integrity and Purity of the Antibody.
Order Now
AVAILABLE SIZES
100Tests
$
526.00
Add to Cart
ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
Data sheet
Download PDF Datasheet
Technical Inquries
Submit Review
Submit Review
Please choose your application, enter the information requested below. * Marked fields are mandatory.
Full Name
Email
Contact Number
Application
Please Select..
Western blot (WB)
Immunohistochemistry (IHC)
Immunofluorescence (IF)
Immunocytochemistry (ICC)
Immunoprecipitation (IP)
Co-Immunoprecipitation (CoIP)
Chromatin Immunoprecipitation (ChIP)
RNA Binding Protein Immunoprecipitation (RIP)
Sample
Loading Amount
Loading Type
UG
CELLS
Treatment
Temperature
Exposure
Observe Band
Fixative
Select One
Acetone
Formaldehyde
Methanol
Paraformaldehyde
Other
Antigen Retrieval
Select One
Heat mediated
Enzymatic
Other
None
Positive Control
Negative Control
Dilution *
1:
Incubation Time *
Select Image *
Choose a file...
Rating *
Remarks *
Submit Review
Product Description
This Nuclear marker antibody is part of a panel of reagents which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. Clone 235-1 antibody specifically detects an antigen associated with the nuclei in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed or frozen tissue sections. The human nuclear antigen antibody can also be used with paraformaldehyde fixed frozen tissue or cell preparations.
Information
Application
FACS, IF
Species Reactivity
Human and Primates. Does not react with mouse, rat and chicken. Other species not known.
Host Species
Mouse
Immunogen / Amino acids
Nuclei of human myeloid leukemia biopsy cells were used as the immunogen for this Nuclear marker antibody.
Conjugation
PE
Clonality
Monoclonal
Clones
235-1
Isotype
Mouse IgG1, kappa
Specifications
Form
Liquid
Storage Buffer
500 ul at 0.1 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Concentration
0.1 mg/ml
Recommended Dilution
Flow Cytometry : 5ul/test/million cells or 5ul/test/100ul of whole blood
Immunofluorescence : 1:50-1:100 for 30 minutes at RTThe concentration stated for each application is a general starting point. Variations in protocols
secondaries and substrates may require the Nuclear marker antibody to be titered up or down for optimal performance.
Misc Information
Storage Instruction
Store the human Nuclear antigen antibody at 2-8°C. Conjugate is light sensitive, store in the dark.
SwissProt
Not Known
Gene ID
Unknown
(human);
References
Protein G affinity chromatography
related data
Get valuable resources and offers directly to your email.
REGISTER NOW