tcna293 Nuclear Antigen Antibody

Data sheet

Download PDF Datasheet

Technical Inquries

Submit Review

Product Description

This nuclear antigen antibody is part of a panel of reagents which recognizes subcellular organelles or compartments. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. Clone 235-1 antibody specifically detects an antigen associated with the human nuclei. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed or frozen tissue sections. The nuclear antigen antibody can also be used with paraformaldehyde fixed frozen tissue or cell preparations.

Information

ApplicationIHC-F, ICC, FACS, IF
Species ReactivityHuman and Primates. Does not react with mouse, rat and chicken. Other species not known.
Host SpeciesMouse
Immunogen / Amino acidsNuclei of human myeloid leukemia biopsy cells were used as the immunogen for this Nuclear antigen antibody.
ConjugationUnconjugated
ClonalityMonoclonal
Clones235-1
IsotypeMouse IgG1, kappa

Specifications

FormLiquid
Storage Buffer0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Concentration0.2 mg/ml
Recommended DilutionIHC (Frozen): 0.5-1ug/ml for 30 minutes at RT (1)
Immunocytochemistry (Acetone-fixed): 0.25-0.5ug/ml for 30 min at RT
FACS: 0.5-1ug/10e6 cells
IF: 0.5-1ug/mlThe concentration stated for each application is a general starting point. Variations in protocols
secondaries and substrates may require the Nuclear antigen antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer
pH 6.0
for 10-20 min followed by cooling at RT for 20 minutes.

Misc Information

Storage InstructionStore the Nuclear Antigen antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
SwissProtNot Known
Gene IDUnknown (human);
ReferencesProtein G affinity chromatography
Get valuable resources and offers directly to your email.